Protan
Protan is a proteinaceous domain derived from the C-terminal part of the lactococcal cell-wall hydrolase AcmA that binds to peptidoglycan (PGN). Mucosis scientists have generated a versatile plug and play toolbox of expression vectors that allow easy insertion of any proteinaceous antigen to produce antigen-Protan fusions in a wide variety of expression organisms, including eukaryotic cells. The use of a secretion peptide (SP) preceding the Ag-Protan fusion allows for the harvesting of the fusion protein from the growth medium (B). Purified Ag-Protan fusions bind instantly to BLPs upon mixing. The binding is non-covalent but highly stable. The resulting antigen-covered BLPs constitute the final Mimopath® vaccine that is delivered to humans via the mucosal layers of the nose (spray) or mouth (capsule), without the need for an injection (C).
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