Publications
Lactococcus lactis as expression host for the biosynthetic incorporation of tryptophan analogues into recombinant proteins. M. El Khattabi, M.L. van Roosmalen, D. Jager, H. Metselaar, H. Permentier, K. Leenhouts, J. Broos. Biochem. J. 409 (2008):193-198.
Neonatal mucosal immunization with a non-living, non-genetically modified Lactococcus lactis vaccine carrier induces systemic and local Th1-type immunity and protects against lethal bacterial infection. K. Ramirez , Y. Ditamo, L. Rodriguez, W.L. Picking, M.L. van Roosmalen, K. Leenhouts, M.F. Pasetti. Mucosal Immunol. 3 (2010):159–171.
Detection and localization of single LysM-peptidoglycan interactions. G. Andre, K. Leenhouts, P. Hols, Y.F. Dufrêne. J. Bacteriol. 190 (2008):7079–7086.
Development of lactococcal GEM-based pneumococcal vaccines. S.A.L. Audouy, S. van Selm, M.L. van Roosmalen, E. Post, R. Kanninga, J. Neef, S. Estevão, E.E.S. Nieuwenhuis, P.V. Adrian, K. Leenhouts, P.W.M. Hermans. Vaccine 25 (2007):2497–2506.
Lactococcus lactis GEM particles displaying pneumococcalantigens induce local and systemic immune responses following intranasal immunization. S.A.L. Audouy, M.L. van Roosmalen, J. Neef, R. Kanninga, M. van Deemter, S. van Selm, G.T. Robillard, K.J. Leenhouts, P.W.M. Hermans. Vaccine 24 (2006):5434-5441.
Immunogenicity of a malaria parasite antigen displayed by Lactococcus lactis in oral immunisations. R. Ramasamy, S. Yasawardena, A. Zomer, G. Venema, J. Kok, K. Leenhouts. Vaccine 24 (2006):3900-3908.
Mucosal vaccine delivery of antigens tightly bound to an adjuvant particle made from food-grade bacteria. M.L. van Roosmalen, R. Kanninga, M. El Khattabi, J. Neef, S. Audouy, T. Bosma, A. Kuipers, E. Post, A. Steen, J. Kok, G. Buist, O.P. Kuipers, G. Robillard, K. Leenhouts. Methods 38 (2006):144-149.
A novel surface display system for proteins on non-genetically modified Gram-positive bacteria. T. Bosma, R. Kanninga, J. Neef, S.A.L. Audouy, M.L. van Roosmalen, A. Steen, G. Buist, J. Kok, O.P. Kuipers, G. Robillard, K. Leenhouts. Appl. Environ. Microbiol. 72 (2006):880-889.
Cell wall attachment of a widely distributed peptidoglycan binding domain is hindered by cell wall constituents. A. Steen, G. Buist, K.J. Leenhouts, M. El Khattabi, F. Grijpstra, A.L. Zomer, G. Venema, O.P. Kuipers, J. Kok. J. Biol. Chem. 278 (2003):23874-23881.